MicroClaveŽ Clear - Drawing Blood Through Your Child's Central Line Using a Syringe
Using a central line to draw blood is a safe, easy way to draw blood samples needed to diagnose and treat your child. This handout explains how you can safely do this once you go home. For young infants, flushing volumes may vary. Please talk with your doctor, nurse, or nurse practitioner.
- Alcohol wipes
- Angel Wing Transfer Device
- 2-3 5 mL syringes
- 1-2 10 mL syringe(s) of saline or ______ mL, as specified
- Blood tubes for samples (color depends on orders)
- 1 5mL syringe of heparinized saline or _______ mL, as specified
Steps Before going home, a nurse will show you how you can safely do these steps once you are home.
1. Wash your hands and put on the gloves.
2. Clean the top of the needleless (MicroClave® Clear) connector with an
alcohol wipe using a “juicing” method for 15 seconds and allow to dry.
3. Using sterile technique, with central line clamped, attach an empty 5 mL
syringe to the needleless connector.
4. Unclamp catheter and pull back slowly on the syringe to draw 3 mL of
“waste” blood into the syringe. This will remove the heparinized saline that
is in the line.
5. Remove the “waste” syringe and attach a new 5 mL syringe.
6. Pull back slowly on the syringe to draw the amount of blood needed to fill the
- If blood does not flow into the tube: Flush the line through the needleless (MicroClave® Clear) connector with 10 mL of saline using a push-stop-push method. Then, repeat steps 1 through 5. If there is still no blood flow, contact your doctor or nurse.
7. Repeat step 5 if more than 5 mL of blood is needed to fill the blood tubes.
8. When finished, remove the syringe.
9. Flush the line through the needleless (MicroClave® Clear) connector with 10
mL of normal saline using a push stop push method (or _____ mL) followed
by 5 mL of heparinized saline (or _____ mL)
After flushing, if you can see blood in the needleless connector, remove the
connector and replace with a new one. See “Changing the Needleless
(MicroClave® Clear) Connector” below.
10. Clamp the line while holding down the plunger and remove syringe at the
11. Remove cap from angel wing transfer device. Attach the syringe filled with
12. Push the blood tube onto the inner needle of the angel wing transfer
device. Blood should automatically fill tube. Blood will not fully fill the
tube. This is normal. Do not try to push more blood into the tube.
13. When blood flow stops, attach next blood tube. If more than 5 ml of blood
is needed always attach the syringe of blood to the angel wing before
you attach the blood tube.
14. When finished, put the angel wing device and all the syringes into a plastic needle disposal box.
Changing the needleless (MicroClave® Clear) connector
Change the needleless connector every three days or if unable to clear it of blood after a blood draw. Note: homecare services may alter this schedule based on home care needs.
1 Remove the plastic cover from the syringe. Push the air out of the syringe.
2 Open the needleless (MicroClave® Clear) connector package and connect the
syringe filled with saline to the end of the needleless connector. Twist on
completely. If the tip of the syringe touches anything other than the end of
the catheter, stop, and replace the syringe.
3 Fill the needleless connector with saline solution by pointing the blue
protective cap found at the other end of the connector up towards the ceiling.
This will remove all air from the connector. Leave the syringe filled with
saline attached to the needleless connector.
4 Clamp your PICC line.
5 Remove the old connector from the PICC line. Hold the line without touching
the catheter opening.
6 Remove the blue protective cap from the needleless connector.
7 Attach the connector to the line. Do not over tighten. One firm turn onto the
line is enough.
8 Unclamp the line.
9 Flush the line using the push stop push method with the remaining flush
solution in the syringe already attached to the connector.
10 Clamp the line while holding down the plunger and remove syringe at the
The information provided should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed physician should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Any duplication or distribution of the information contained herein is strictly prohibited.
Last Updated: 08/06/2012
Copyright © 08/06/2012 University of Wisconsin Hospitals and Clinics Authority. All rights reserved. Produced by the Department of Nursing. HF#5888
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